Composite
Part:BBa_K1583107:Design
Designed by: Stefan Robert Marsden Group: iGEM15_TU_Delft (2015-09-08)
pRha + CsgA with His-tag & pTet + RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 635
Illegal AgeI site found at 747 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
Source
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).